anti-rabbit regiiiγ  (ABclonal Biotechnology)

Journal: eBioMedicine

Article Title: Intestinal epithelium dysfunctions cause IgA deposition in the kidney glomeruli of intestine-specific Ap1m2 -deficient mice

doi: 10.1016/j.ebiom.2024.105256

Figure Lengend Snippet: Loss of AP-1B decreases IL-22 - STAT3 signal, leading to decreased RegIIIγ expression in the intestine . (A) Western blot analysis of pSTAT3, STAT3, and β-actin in the intestinal epithelium from Ap1m2 fl/fl and Ap1m2 ΔIEC female mice. n = 4. (B) Quantitative PCR analysis of Reg3b and Reg3g in the intestine from ileal Ap1m2 fl/fl and Ap1m2 ΔIEC female mice. Tbp was analyzed as a reference gene. n = 4. (C) Immunofluorescence images of RegIIIγ (red) and nuclei (blue) in the intestine from Ap1m2 fl/fl and Ap1m2 ΔIEC female mice. Scale bars: 100 μm. Data represent the median. ∗ p < 0.05 (unpaired, two-tailed Mann–Whitney test).

Article Snippet: The primary antibodies were as followed: rabbit anti-C3 (1:200, Cat. No. A006302, DAKO, Carpinteria, CA, RRID: AB_578478 ), rat anti-mouse CD107a (LAMP-1) (1:200, clone 1D4B, BioLegend, San Diego, CA, RRID: AB_572020 ), goat anti-mouse IgA (1:200, Cat. No. A90-103A; Betyl Laboratories, RRID: AB_67136 ), rat anti-mouse IgA (1:200, clone RMA-1, BioLegend, RRID: AB_315076 ), Alexa Fluor 488-conjugated donkey anti-mouse IgG (1:400; Cat. No. 715-545-150, Jackson ImmunoResearch, West Grove, PA, RRID: AB_2340846 ), goat anti-mouse pIgR antibody (1:200, Cat. No. AF2800, R&D Systems, Minneapolis, MN, RRID: AB_2283871 ), and rabbit anti-RegIIIγ (1:200, Cat. No. ab198216, Abcam, RRID: AB_2892581 ).

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Immunofluorescence, Two Tailed Test, MANN-WHITNEY

Journal: Annals of surgery

Article Title: The Enteric Nervous System (ENS) Neuropeptide, Bombesin (BBS), Reverses Innate Immune Impairments during Parenteral Nutrition (PN)

doi: 10.1097/SLA.0000000000000871

Figure Lengend Snippet: Specific primers used for PCR assay.

Article Snippet: Membranes were then incubated with primary antibody, monoclonal lysozyme (ab108508, Abcam) diluted 1:5000 and Rabbit polyclonal RegIII-γ (PA5-25517, Thermo Scientific) diluted 1:250 overnight at 4°C with constant agitation.

Techniques:

Journal: Annals of surgery

Article Title: The Enteric Nervous System (ENS) Neuropeptide, Bombesin (BBS), Reverses Innate Immune Impairments during Parenteral Nutrition (PN)

doi: 10.1097/SLA.0000000000000871

Figure Lengend Snippet: IHC of sPLA2 in A) Chow-fed mice; B) PN-fed mice; C) PN+BBS-fed mice. IHC of lysozyme in D) Chow-fed mice; E) PN-fed mice; F) PN+BBS-fed mice. IHC of RegIII-γ in G) Chow-fed mice; H) PN-fed mice; I) PN+BBS-fed mice. Images are 20X magnification of intestinal villi and crypts. Staining for sPLA2 and lysozyme is decreased in PN-fed mice and appears qualitatively similar in Chow and PN+BBS-fed mice. RegIII-γ staining appears cytosolic and localizes around intestinal villi. PN, parenteral nutrition; BBS, bombesin.

Article Snippet: Membranes were then incubated with primary antibody, monoclonal lysozyme (ab108508, Abcam) diluted 1:5000 and Rabbit polyclonal RegIII-γ (PA5-25517, Thermo Scientific) diluted 1:250 overnight at 4°C with constant agitation.

Techniques: Staining

Journal: Annals of surgery

Article Title: The Enteric Nervous System (ENS) Neuropeptide, Bombesin (BBS), Reverses Innate Immune Impairments during Parenteral Nutrition (PN)

doi: 10.1097/SLA.0000000000000871

Figure Lengend Snippet: A) Effects on ileal tissue RegIII-γ mRNA (n=7 per group); B) effects on ileal tissue RegIII-γ relative protein concentration (Chow: n=12; PN: n=10; PN+BBS: n=11); C) effects on RegIII-γ protein in intestinal wash fluid (Chow: n=11; PN: n=12; PN+BBS: n=9); and D) effects on RegIII-γ release after cholinergic stimulation with bethanechol (Chow: n=10; PN: n=12; PN+BBS: n=9). Data are presented as mean ± SEM. *p<0.05 versus Chow; #p<0.05 versus PN. PN, parenteral nutrition; BBS, bombesin.

Article Snippet: Membranes were then incubated with primary antibody, monoclonal lysozyme (ab108508, Abcam) diluted 1:5000 and Rabbit polyclonal RegIII-γ (PA5-25517, Thermo Scientific) diluted 1:250 overnight at 4°C with constant agitation.

Techniques: Protein Concentration

Journal: Annals of surgery

Article Title: The Enteric Nervous System (ENS) Neuropeptide, Bombesin (BBS), Reverses Innate Immune Impairments during Parenteral Nutrition (PN)

doi: 10.1097/SLA.0000000000000871

Figure Lengend Snippet: Mechanism of innate defense for studied antimicrobial peptides.

Article Snippet: Membranes were then incubated with primary antibody, monoclonal lysozyme (ab108508, Abcam) diluted 1:5000 and Rabbit polyclonal RegIII-γ (PA5-25517, Thermo Scientific) diluted 1:250 overnight at 4°C with constant agitation.

Techniques: Activity Assay, Lysis